Michael Hooker Microscopy Facility (MHMF.ORG)

Leica Confocal Files - Images are tiff.  Primary acquired data are indexed (8 bit) tiff images.  Overlays or other images saved with "right click -> save image -> raw" are RGB 8 bit tiff images

• Use Leica LCS software on confocal scanning computer (\\Laplace) or WinodwsXP image processing work stations (\\Nomarski or \\Snell)
• Use Leica LCS-lite software - install on your PC running WindowsXP or WindowsNT4 (does not run on Windows 2000, Vista or 7)

Zeiss Confocal Files - Raw data is a Zeiss version of multi-page tiff which is not easily opened with general purpose image processing software.  Raw data is generally 8 or 12 bit indexed images

• Use Zeiss LSM software on confocal scanning computer (\\Hertz) or image processing work stations (\\Snell)
• Use Zeiss Image Browser - install on your own PC

C-Imaging - Images are 8 or 16 bit indexed for raw images.  Overlays are RGB format

• Use overlay/merge functions and save using "save all channels"

Photoshop

• Convert indexed images to either 8 bit gray scale or RGB
• Merge - there are several ways of doing this:
  • Overlay method: Overlaying red/green images using Photoshop
  • Layer method:

		Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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