Michael Hooker Microscopy Facility (MHMF.ORG)

Scale Bars / Calibration

• General Scale Bar Considerations

• Adding Scale bars:
  • Leica confocal images
  • Zeiss confocal images
  • Widefield scopes - Please note:
    • 1. The objective used (knowing NA can be helpful)
    • 2. Intermediate zoom (usually 1x)
    • 3. Camera used
    • 4. Camera binning factor
  • C-Imaging
  • Metamorph
  • Image-J
  • Volocity
  • Photoshop
  • Act-1

   

• Micrometer Images for specific microscope systems:
  • Nikon Microphot SA Upright (\\Hooke)
  • Leica DMIRB inverted (\\Fresnel)
  • Nikon TE2000 inverted scope (\\Morpheus)
  • Nikon TE2000 inverted scope (\\Nipkow)
  • Leica MX16FA stereo micro/macro scope

		Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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