Michael Hooker Microscopy Facility (MHMF.ORG)

Leica MZ16FA Fluorescence Stereo Microscope/Macroscope

The System:

• The Leica MZ16FA Fluorescence Stereo Microscope/Macroscope in detail

Quick Guide:

Powering Up the System:

• Note that the two microscope switches and the b/w camera must be on before the software is started
• If doing fluorescence: Leica EL6000 metal halide lamp
  • Turn on green power switch
  • Ensure shutter switch is pushed in.  Yellow shutter open light should be on
• MZ61FA (microscope stand)
  • Turn on switch at back of base on right side
  • Turn on switch on top of focus drive
• Ensure computer is on
• Ensure monitor is on
  • Ensure video cable on back of monitor is from the computer and not the VCR
  • If you use the VCR please put the computer's video connector back when your done!
• Turn on Hamamatsu OrcaER camera
  • Ensure the Hamamatsu OrcaER is the camera on the top left c-mount port on the scope body.
  • If not -
    • loosen locking screw
    • pull out little video camera and c-mount tube
    • unscrew c-mount
    • unscrew cap that should be on OrcaER
    • screw cap into small video camera
    • attach c-mount to OrcaER
    • insert OrcaER and c-mount into top left port on microscope body
    • tighten locking screw
    • Users of the video camera should the OrcaER camera back into position
  • Ensure camera cooling is enabled
• Turn off or on incident light lamp - KL1500 (Schott) - if required.
• Log in to computer using your user name, password and the MHmicroscopy domain
• Start C-Imaging
• Turn off transmitted light by pushing button on the rear of the right side of the base
• Change filters on scope body if necessary & run scope hardware setup on tab in acquisition window of c-imaging

Taking Pictures (real brief notes)

• Select camera
• Select channel(s) and filters
• Go live
• Set exposures and gains for each channel
• If transmitted light - do a white balance on a white/blank
• Press stop then capture1 in order to snap a picture
• Remember to save pictures

Powering Down the System:

• Exit C-Imaging (don't forget to save any pictures)
• Power off at top of focus drive
• Power off scope body switch at rear right side of base
• Turn off Leica EL600 metal halide lamp
• Please sign log book

		Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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