Michael Hooker Microscopy Facility (MHMF.ORG)

Draft Notes!
Very Basic MultiTime Time Lapse on the Zeiss 510 meta Confocal

When doing large time lapse scans where system memory will be taxed and image file saving is unreliably use the more complex MultTime Macro.

1. First Time Use

You will need to have a macrobutton linked to the latest version of MultiTime.

• Assign button 10 to MultiTime version 32
• Delete any other buttons linked to (probably the older) MutliTime

2.1 Basic Time Lapse (single or multiple simultaneous channel scanning (Single Track))

• Close Time Series window (just to be safe)
• Setup z-series scanning or single slice scanning in the normal way. 
• In the Configuration Control window save the setup by pressing the Config button and storing the setup.  Give it a unique name and allow overwrite if necessary.  Always resave the configuration anything is changed include gains, offsets, pinhole size, top and bottom z-positions for z-series, laser powers, AOTF settings, filters, etc., etc., etc.
• Close MultiTime Series window if it is open
• In LSM 510 - Expert Mode window push the Macro button and choose MultiTime
• Setup for time lapse:
  • Fixed Location
  • Keep the single block 1
  • Set Experiment Repetitions
  • Keep Views at 1 and Group repetitionss at 1
  • Set a suitable wait delay (time between end of a scan and start of next)
  • Choose Single track (XY)
  • Make sure ZStackXY is checked
  • Press Load Conf
  • Enter a base name
  • Select Image DB
  • Pres Update
  • To go press Start Time

2.2 Variable Time Interval Time Lapse Acquisition including FRAP

• Setup a simple time-lapse using "EditBleach" and "TimeSeries"
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Last updated 2009-06-02