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Michael Hooker Microscopy Facility (MHMF.ORG) |
Antibody Staining
Alexa Fluors
Alexa Fluor dyes (which are anionic) sometimes exhibit nonspecific background due to electrostatic binding to polycations (e.g. PLL). Image-iT FX signal enhancer (Mol. Probes catalog number I36933)is a blocking agent that is quite effective in reducing this background. Commercial interest acknowledged etc.
Iain Johnson Molecular Probes/Invitrogen
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Copyright 2001-2014 Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599 |
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Last Updated: 2014-07-23 |