Michael Hooker Microscopy Facility (MHMF.ORG)

An expanded version of this hands on course will be run again in the Fall of 2007

Microscopy Facility Tutorial Workshop - Light Microscopy for Biomedical Research 2006
Monday - Dec 4	Location	Tuesday - Dec 5	Location	Wednesday - Dec 6	Location	Thursday - Dec 7	Location	Friday - Dec 8	Location
9:00 Intro.  Brief History of Light Microscopy, modes of imaging, Parts of a Light Microscope - M. Chua	TB7033 (9-11) no food permitted in room	9:00 Talk - Fluorescence, why use it, Jablonski diagram, contrast - W. Salmon	TB3200 (9-11) food OK	9:00 Talk - Confocal Microscopy - M. Chua	TH124 (9-11) food OK	9:00 Talk - Advanced confocal, multichannel fluorescence, 2 photon, spinning disk - M. Chua	TB3200 (9-11) food OK	9:00 Talk - CCD cameras, PMT, noise, color/monochrome  - M. Chua	TB7033 (9-11:30) no food permitted in room
Break		Break		Break		Break
10:00  Objectives, Kohler illumination, image formation 11:00 Break	TB7033 (9-11) no food permitted	10:00 Talk - Filters, Immuno labeling, cross talk, live cell imaging - M. Chua	TB3200 (9-11) food OK	10:15 Confocal continued - M. Chua	TH124 (9-11) food OK	10:00 Talk - Digital images, pixel depth, sampling, formats, pitfalls, resolution, Nyquist - M. Chua	TB3200 (9-11) food OK	10:15 Break
11:15 Walk through of MHMF - details of each machine	TB6129	11:00 Hands on - Fluorescence + Kohler review	TB6129	10:45 Hands on - confocal I, sensitivity, pinhole, filters, averaging, zoom pixel size		11:00 Hands on - digital image handling, calibration, contrast, time-lapse		10:30 Talk - Brief introduction to: co-localization, deconvolution - M. Chua	TB7033 (9-11:30) no food permitted in room
12:00 Lunch break		12:00 Lunch break		12:00 Lunch break		12:30 Lunch break		11:30 Lunch break
13:00 Hands on - parts of a microscope, care, digital acquisition		13:00 Invited Talk - Klaus Hahn: Fluorescent Biosensors for Living Cells	TB3200 (1:00-2:00) food OK	13:00 Hands on - confocal II, z-series, time lapse		13:30 Hands on Confocal multi-channel,  FRAP, spectral scanning(?)		12:30 Hands on - movies, scaling compression, resizing, conversion, format for Power Point 13:45 Sum up
15:00 Invited talk - Ted Salmon: DIC/Phase Contrast	TB3200 (3:00-4:00) food OK	14:00 Hands on - time lapse		~14:30 Tutorial - Rendering, 3D Hands on - 3D projection	TB6129	15:30  Invited talk - Ken Jacobson: Fluorescence and FRAP	TB7033 (3:30-4:30) no food permitted in room	14:00 Invited talk - Franck Polleux: Tracing cortical projections	TB3200 (2:00-3:00) food OK

Contacts: Michael Chua  843-3268, Wendy Salmon  966-7051, Karen Stone  966-9431 Office of Research, School of Medicine

Instructors:
Allan Fanning, Neal Kramarcy, Wendy Salmon, Michael Chua, Juergen van der Heiden