Michael Hooker Microscopy Facility (MHMF.ORG)
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Michael Hooker Microscopy Facility (MHMF.ORG) |
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Light Microscopy for Biomedical Research - Workshop September 24 to 28, 2007
The Michael Hooker Microscopy Facility, in collaboration with the North Carolina Roadmap Initiative and the UNC-Chapel Hill School of Medicine, is offering a one week workshop on the use of light microscopy in biomedical research. The workshop is intended to provide an introduction to the basic concepts of light microscopy and its application to biological research with lectures, hands on sessions and industry involvement.
The 2007 workshop is over. You may submit your information at Application Form if you wish to be added to the wait list.
Tuition and Tuesday dinner: $700. A limited number of the seats are reserved for those who are members of groups/labs. that already support the MHMF through specific grants. For more information contact Michael Chua lmbr-mc@mhmf.org
| Microscopy Facility Workshop - Light Microscopy for Biomedical Research 2007 - This schedule is subject to change | |||||||||
| Monday - Sept 24 | Location | Tuesday - Sept 25 | Location | Wednesday - Sept 26 | Location | Thursday - Sept 27 | Location | Friday - Sept 28 | Location |
| 9:00 Intro. - History of Light Microscopy, Modes of imaging, Parts of a Light Microscope - M. Chua |
9-12 |
9:00 Talk - Fluorescence: Jablonski diagram, contrast, filters, cross talk - W. Salmon |
9-12 |
9:00 Talk - Confocal Microscopy I - principle, scanning - M. Chua |
8:30-10:30 |
9:00 Talk - Advanced confocal, 2 photon, spinning disk, colocalization, digital images - M. Chua | 09-10 |
9:00 Invited Talk - 3-D Image Deconvolution, 3-D Visualization - Katie Buchanan | 9-10:30 |
| 10:15 Break 10:30 | 10:15 Break 10:30 | 10:15 Break 10:30 | 10:15 Break 10:30 | 10:15 Break 10:30 | |||||
| 10:30 Objectives, Kohler illumination, Image formation | 9-12 |
10:30 Invited Talk - Immuno labeling, cell labeling Kelly Lundsten |
9-12 |
10:30 Confocal II continued - PMT, multichannel, 3d, projections | 10:30-12 |
10:30 Invited Talk - Fluorescence and FRAP - Chris Baumann | 10:30-12 |
10:30 Image Deconvolution, Tracking, 3-D rendering | LAB 10:30-12:30 |
| 11:45 Lunch break | 11:45 Lunch break | 11:45 Lunch break | 11:45 Lunch break | 12:30 Lunch break | |||||
| 12:45 Hands on - parts of a microscope, care, digital acquisition - alignment | LAB | 12:45 Hands on - Fluorescence | LAB | 12:45 Hands on Confocal I - sensitivity, pinhole, filters, averaging, zoom pixel size z-series, time lapse, simult/sequen. Coloc. | LAB |
12:45 Hands on Confocal II - multi-channel, FRAP, ) Rendering, 3D,
projections 3-D Deconvolution |
LAB | 1:30 Invited Talk - Visualization and Representation - Dr. Russell Taylor |
1-2:30
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| 15:30 Invited Talk - DIC/Phase Contrast - Dr. Ted Salmon | 15-17 |
15:30 Invited Talk - 1. CCD cameras, noise, color/monochrome Mike Meade 2. Digital images, sampling, resolution, Nyquist - M. Chua |
15-17 |
15:30 Invited Talk - Fluorescent Biosensors for Living Cells - Dr. Klaus Hahn | 15:30-17 |
15:30 Invited Talks - |
15:30-17 |
14:30 Hands on - movies, scaling compression, resizing, conversion, format for Power Point | LAB 15-17 |
| Finish 5 PM | Finish 5 PM Dinner 6:30 PM |
Finish 5 PM | Finish 5 PM | Finish 5 PM | |||||
Faculty includes: Ted Salmon, Klaus Hahn, Katie Buchanan, Frank Herbert, John Miles, Larry Kordon, Hilary Hicks, Chris Freson, Dave Tilley, Juergen von der Heiden, Lennie Cleaton, Kelly Lundsten, Janine Babulski, Katie Buchanan, Elise Shumsky, Mike Meade, Chris Baumann, Russell Taylor
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