Michael Hooker Microscopy Facility (MHMF.ORG)

Hardware Resources

• FLUORESCENCE MICROSCOPES (Wide field)
  • Inverted Leica fluorescence microscope with digital B/W and color cameras and DIC
  • Upright Nikon fluorescence microscope and a dissecting microscope with a high resolution color CCD camera
  • Inverted Nikon TE2000 fluorescence, time lapse, multiple fluorescence, DIC, Phase Contrast, ratio imaging, Fura-2, Prim, CFP/YFP, etc.
  • Leica Fluorescence Stereo Micro/Macrosope
  • Nikon Upright fluorescence and transmitted light microscope (Eclipse 80i) with color camera (DIC, phase contrast, fluorescence)
• CONFOCAL MICROSCOPES - Comparison of confocal features - another comparison
  • Spinning Disk Confocal, Perkin Elmer, Ultraview LCS
  • Laser Scanning Confocal Microscope Leica SP2 UV aobs
  • Laser Scanning Confocal Microscope Zeiss 510 meta
  • Laser Scanning Confocal Microscope Olympus FV1000 multiphoton
• MISCELLANEOUS
  • Image analysis and quantification workstations - Network names: Normaski - Brewster - Snell - Zernike - Schott - Young
    • Volocity 6 - visualization - quantification - deconvolution - remote license server
    • Metamorph 7 - off line
    • Zeiss 510 - LeicaLCS 2.6 - C-Imaging 6 with deconvolution
    • Adobe Creative Suite - Photoshop - Illustrator - Acrobat - Premiere
    • MS Office 2003/7/10 - Word - Excel - Power Point - FrontPage
    • QuickTime 7
    • VidEdit
    • VHS video digitization
    • Flatbed scanner - reflectance and transparency
  • Laser Micro-Dissection and capture - currently unavailable
  • Atomic Force Microscope - limited support
  • Laser jet double sided printer: HP1320
  • Incubator 37 C, 5% CO2 and 95% controlled humidity
  • Refrigerator (4 C) for sample or reagent storage
  • Heated stages, objective heater, 5% CO2
  • Sonicator
  • Digital Oscilloscope (100 MHz)
  • Laser Power Meter (Coherent )
  • Soldering iron
  • Tools
  • Multimeter & Clamp around current meter

Software Resources

• Volocity 6  3D rendering, classification  and deconvolution license on \\Schott  & \\Nomarski  &  \\Snell  & remote license server
• Metamorph 7 - \\Nomarski  &  \\Snell
• Zeiss LSM 3.2 - full display & analysis software on \\Snell
• Zeiss Image Browser 3.2 - available on CD or download
• LeicaTCS off line with complete analysis  - \\Snell  \\Nomarski
• LeicaTCS light  - available on CD or download
• Nikon Elements - \\Hamilton
• ImageJ - \\Lister \\Fresnel
• PhotoShop 7 to CS5 Master collection on \\ Nomarski,  \\Schott,  \\Fresnel,  \\Hooke,  \\Young, \\Snell
• Premiere 6.0 on \\Young
• C-Imaging analysis - \\Nomarski,  \\Schott,  \\Snell  and also on acquisition stations: \\Fresnel, \\Hooke, \\Morpheus, \\Gould, \\Euler, \\Shiva, \\Wheatstone, \\Nipkow
• Winzip 14.5  -  10 computer license
• ThumbsPlus 7 site license

Techniques Available

• Laser Scanning Confocal:
  • 4-color fluorescence + DIC = 5 channel imaging, Visible & UV excitation
  • Fluorescence Recovery After Photobleaching (FRAP) up to 80 fps with small images
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fast X-Z scanning
  • 3D &/or time lapse image acquisition
  • Spectral Imaging – dye unmixing
  • Co-localization
  • Time lapse
• Live Cell Imaging:
  • 3-color Spinning-disk Confocal (FITC, Texas Red & CY5 like dyes)
  • Computerized control of light exposure
    Simultaneous fluorescence and transmitted light (DIC)
  • Heated stages
  • Temperature and environmental control
• Advanced and Standard Widefield microscopy:
  • Ratio imaging on an inverted microscope, e.g. Ca²+ Fura-2
  • Dual camera imaging
  • Phase contrast and DIC/Nomarski
  • Time-lapse (fluorescence and transmitted light)
  • Intensified CCD camera for high sensitivity
• Color brightfield imaging (high resolution):
  • Nikon DXM 1200 pixel shift color camera (11 MegaPixels)
• Image Processing and Analysis:
  • Co-localization
  • Particle tracking
  • 3-D Image rendering
  • Fluorescence quantification
  • Densitometry
  • Area measurements
  • Time lapse analysis
  • Deconvoluion
  • Presentation preparation

How Tos

Go to How To's

Planned Resources

• Whole embryo imaging
• Multiphon confocal FLIM

Planned Resources currently shelved

• FCS
• TIRF

		Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
Go Back	Booking Resources	Questions/comments/problems: Michael Chua